IGEM:Peking/2007/Count-Conjugation-Notebook/2007-10-14

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Transformation:F-promoter-E0240(into dH5a) and R-OriT(into R751-delect)

double digestion for ligation:B0015-ROriT

ligation: B0015-ROriT<-E0240 and FtraI->pSB1A2

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