IGEM:Peking/2007/Switch-Notebook/2007-7-29
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CD2-T send to sequencing
after colony PCR and double digestion test result is right
Rescue of Pla-T1T2, Pla-T1TE by electrophoresis
test result after rescue: good with high concentration
positive transformation of plx007/008/009 and RD2-T
ligation of RD2-pGEM-T
transformation and smear onto plate
rescue by precipitation
T1T2 and T1TE, rescue after inactivation
checking by electrophoresis to see there is a slight band
ligation of T1T2 to T1TE
self-ligation as a control
Colony PCR of T1T2-P, T1TE-P and T1T2-pcc009
ddH2O 11uL
buffer 1.5uL
dNTP 1.5uL
primer F/R 0.5uL each
ExTaq 0.1uL
total 15uL
result: we got T1TE-P right!