IGEM:Peking/2007/Switch-Notebook/2007-9-6

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Contents

Check our plate

By smearing competent cells directly onto plates without any transformation procedure, we can check whether the plates with Kanamycin is right.

Result: The plates have some thing wrong and we discarded them!

Colony PCR of I7100 ligation product

Fail!

I7100 digestion test

using H buffer, PstI/EcoRI

Fail!

Digestion of cIind-

digestion another time of vector and fragments

using still PstI/EcoRI and H buffer

Miniprep of I7100 for the second time

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