Check our plate
By smearing competent cells directly onto plates without any transformation procedure, we can check whether the plates with Kanamycin is right.
Result: The plates have some thing wrong and we discarded them!
Colony PCR of I7100 ligation product
I7100 digestion test
using H buffer, PstI/EcoRI
Digestion of cIind-
digestion another time of vector and fragments
using still PstI/EcoRI and H buffer