IGEM:Peking University/2008/Experiment/PKU 0603 G2 1

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PCR

TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6
Volume (μL)
Template (Budding Yeast Genome)1
dNTP2
Enzyme0.5
Buffer2
F-Gal4-HindIII-ApaI 0.5
R-Gal4-EcoRI-Xhol0.5
ddH2O13.5
conditions
Temperature(&&&)Time Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C00:304 cycles
94°C01:0030 cycles
52.0,53.6,56.1,58.8,60.1,62.0°C02:0030 cycles
72°C04:0030 cycles
72°C05:00
4°CHold


TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3
Volume (μL)
Template (Budding Yeast Genome)1
dNTP5
Enzyme1
Buffer5
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol1
ddH2O36
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C00:304 cycles
94°C01:0030 cycles
54.7,57.4,60.1°C02:0030 cycles
72°C04:0030 cycles
72°C05:00
4°CHold


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