IGEM:Peking University/2008/Experiment/PKU 0605 G2 1

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Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
Volumn(μL)
pUC18 5
HindIII0.2
EcoRI0.2
M Buffer2
ddH2O12.6

Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
Volumn(μL)
Gal415
HindIII0.2
EcoRI0.2
M Buffer5
ddH2O29

Ligation-pUC18/Gal4
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