IGEM:Peking University/2008/Experiment/PKU 0807014 G1 1

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PKU_080714_G1_1


Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)

Enzyme cutting-pADH-(lacS/tet)(PCR product)
Volume (μL)
pADH-(lacS/tet)14
SacI2
XhoI2
M Buffer10
ddH2O72


Enzyme cutting-pGREG504-1
Volume (μL)
pGREG504-14
SacI2
XhoI2
M Buffer10
ddH2O82


lanes:lacS|tet|vector|marker1|marker2


Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)5
SacI1
XhoI1
M Buffer2
ddH2O11


Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2)5
SphI0.2
XhoI0.2
H Buffer2
ddH2O12.6


Volume (μL)
pGREG504-pADH-(Control1/Control2/Control3)5
BamHI0.2
XhoI0.2
K Buffer2
ddH2O12.6
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