IGEM:Peking University/2008/Notebook/Group 1/2008/05/14

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PKU_080514_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP)1
easytaq mixture25 *3
F-Primer0.5
R-Primer0.5
ddH2O23


conditions
Temperature(°C)Time Length(0:00:00)
9405:00
9400:3030 cycles
50.1,52.2,55.1,58.3,62.201:0030 cycles
7201:3030 cycles
7207:00
Alarm5 times
Hold4°C



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