IGEM:Peking University/2008/Notebook/Group 1/2008/07/17

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PKU_080717_G1_1


PCR- pGREG504-pADH-(lac1/lac2/lacS/tet)-50ul
Using program lac12MQT

Enzyme cutting-pADH-(lac1/lac2/lacS/tet)(PCR product)
Volume (μL)
Promoter15
BamHI2
SacI2
0.5×K Buffer5
ddH2O76


Volume (μL)
Promoter15
SacI5
XhoI5
1×M Buffer10
ddH2O65



Enzyme cutting-AID
Volume (μL)
AID15
BamHI2
XhoI2
1×K Buffer10
ddH2O71



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