IGEM:Peking University/2008/Notebook/Group 1/2008/08/16

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PKU_080816_G1_1

Purification PCR product-f1, f2, f3, f4, P1, P2, P3, P4




Enzyme cutting 50μL-504-AID-lexA 10μL-SacI 2μL-BamHI 0.5μL


Enzyme cutting 50μL-504-AID-lexA 20μL-BamHI 1μL-XhoI 1μL


Enzyme cutting 50μL-TSH's plasmid 10μL-BamHI 1μL-XhoI 1μL



Heat killing enzyme-65°C-20min


PCR purification-P1, P2, P3, P4, TSH, 504(4)


Gel extraction-504(2) and purification 800bp



Ligation P1-4 - 504(4)


Ligation 504(2)-TSH


Gel running and checking-ligation product



Transformation



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