IGEM:Peking University/2008/Notebook/Group 2/2008/06/07

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0607

TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*6
Volume (μL)
Template (Budding Yeast Genome)1
dNTP5
Enzyme1
Buffer5
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol1
ddH2O36
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C00:304 cycles
94°C01:0030 cycles
49.2,52.6,54.6,56.5,58.2,59.8°C02:0030 cycles
72°C04:0030 cycles
72°C05:00
4°CHold

Enzyme cutting-pUC18&Gal4-HindIII-EcoRI-20 μL
Volumn(μL)
pUC18 5
HindIII0.2
EcoRI0.2
M Buffer2
ddH2O12.6



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