IGEM:Peking University/2008/Notebook/Group 2/2008/06/27

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0627

TITLE: PCR-pPT-Gal4-Transtart 20ul*6
Volume (μL)
Template0.5
dNTP2
Enzyme0.5
Buffer2
F-Primer0.2
R-Primer0.2
ddH2O14
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C05:004 cycles
94°C01:0030 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C01:0030 cycles
72°C03:0030 cycles
72°C10:00
4°CHold

Enzyme cutting-T-Gal4-XbaI-20*6 μL
Volumn(μL)
DNA2
XbaI1
M Buffer2
BSA2
ddH2O13

Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL
Volumn(μL)
DNA2
SpeI1
HindIII1
M Buffer2
ddH2O14



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