0627
TITLE: PCR-pPT-Gal4-Transtart 20ul*6
|
Volume (μL)
|
Template |
0.5
|
dNTP |
2
|
Enzyme |
0.5
|
Buffer |
2
|
F-Primer |
0.2
|
R-Primer |
0.2
|
ddH2O |
14
|
- conditions
Temperature |
Time Length(0:00:00)
|
94°C |
02:00
|
94°C |
01:00 |
4 cycles
|
50°C |
02:00 |
4 cycles
|
72°C |
05:00 |
4 cycles
|
94°C |
01:00 |
30 cycles
|
55.2,55.6,57.6,59.5,61.3,63.9°C |
01:00 |
30 cycles
|
72°C |
03:00 |
30 cycles
|
72°C |
10:00
|
4°C |
Hold
|
- Enzyme cutting-T-Gal4-XbaI-20*6 μL
|
Volumn(μL)
|
DNA |
2
|
XbaI |
1
|
M Buffer |
2
|
BSA |
2
|
ddH2O |
13
|
- Enzyme cutting-T-Gal4-SpeI-HindIII-20*4 μL
|
Volumn(μL)
|
DNA |
2
|
SpeI |
1
|
HindIII |
1
|
M Buffer |
2
|
ddH2O |
14
|
|