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August 10, 2007

  • Luc did miniprep on P1010
  • I ran a digest with SpeI and EcoR1 again
  • I used PCR tubes this time and ran 20uL digests
  • Added to tubes:
    • 4,8,and 12uL DNA
    • 2uL EcoR1 buffer
    • 2uL BSA
    • 0.5uL each restriction enzyme
    • dH2O to yield final volume of 20uL
  • Started incubating at 12:50pm


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