IGEM:PennState/Labbook/NoahJohnson/2007-8-2
From OpenWetWare
Jump to navigationJump to search
August 2, 2007
- Ordered UV cuvettes
- Began growing up 6 3ml cultures of crp* in LB
- Will TELT dna prep them tonight or tomorrow
M9 Minimal Media (250mL each):
For ~250mL of media and a final 0.2% sugar concentration:
- 200mL sterile water
- 50mL of 5X M9 salts
- 12.8g Na2HP04*7H2O
- 3g KH2PO4
- 0.5g NaCl
- 1g NH4Cl
- Dissolve in 200mL deionized water and autoclave to sterilize
- 3.75g BactoAgar
Autoclave to sterilize and allow to cool until you can hold it for 2 seconds on the palm of your hand. Then add the following and pour into plates:
- 25mL sugar solution
- Add 0.5g glucose/xylose to 25mL sterile water
- Filter-sterilize before adding
- Makes a 12.5% sugar solution and a 0.2% overall glucose/xylose media
- 500 uL 1M MgSO4
- Dissolve 24.65g MgSO4*7H20 in 100mL H20
- Autoclave to sterilize
- 25uL 1M CaCl2
- Dissolve 14.7 g CaCl2*2H2O in 100mL H2O
- Autoclave to sterilize
I will make 250mL of each of the following:
- 0.2% glucose
- 0.2% glucose + 0.2% xylose
- 0.2% xylose
- 0.2* glycerol (- control)
- DNA prep on crp* cultures from this morning using TELT method
10-