IGEM:PennState/Labbook/NoahJohnson/2007-8-20
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August 20, 2007
PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase)
Restriction digest of crp* and P1010 in psB1A2 with EcoR1 and SpeI
Ran gel of restrictions:
- All 4 crp* digests worked
- none of the psB1A2 digests seemed to work
10-6:30