IGEM:PennState/Labbook/NoahJohnson/2007-8-21
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August 21, 2007
- Set up 20uL double digests with psB1A2 as well as sequential digests with EcoR1 and SpeI
- Used MP from galen's box marked:
- 6B for E+S
- 4B for E
- 3B for S
- Started incubating at 11:03am
- Ran gel on restriction products
- Started @ 1:50pm
- Started an overnight ligation at 16C with old crp* 4uL restriction E+S from yesterday and the gel extracted psB1A2 restriction E+S from 8/10
- Used a 6:2 ins:vect ratio
9:30-6:30