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September 16, 2007

  • Started overnight ligation with crp* and psB1A2 E+S restrictions
  • Used a 6uL:2uL ins:vect ration
  • Used PCR tubes and 7.86pH dH20
  • Left 2 ligations in the 16C fridge and 2 ligations on the benchtop in ice to melt overnight with a negative for each
  • Innoculated starter culture of DH5a for competent cell prep tomorrow morning


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