IGEM:Penn State University 2006/calendar/2006-6-27
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To Do: Tuesday
Tony
- restrict and run gel pSB4A3 (need more volume than yesterday)
- gel extract, ligate, and transform IIR+motB constructs into pSB4A3
- competency prep on RP437
- transform RP437 and RP3087 with GFP
- transform R0100+motB, C0051 (from registry plate) and pSB4A3 into DH5a
Jon
- use Pat's program to create a map of the IIR+motB part in pSB1A2 and pSB4A3
- restrict R0051 and motB, and run on gel
- make LB AND SOB
Chris
Joel
- Inoculate 6ml of inducible, 2ml constitutive, 2ml DH5-alpha at 11AM.
- Plate reading on 9hr cultures at 8PM and 24 hr cultures at 10PM.
- Read Drew Endy's plate reading assay protocals Endy lab page
- inoculate 2ml of inducible (I14033 and I14032) and 2ml of constitutive.
Ralph
- Plate read (Pat's Lab) see above
- Read Drew Endy's plate reading assay protocals Endy lab page
Lane
- Innoculate colonies from pLacIq+B0030/31/34+C0012 plates
- ?competency prep on RP437?
- Transform I14032+E0240 into RP437
Deepti
- stuff