IGEM:Penn State University 2006/calendar/2006-6-27

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Contents

To Do: Tuesday

Tony

  • restrict and run gel pSB4A3 (need more volume than yesterday)
  • gel extract, ligate, and transform IIR+motB constructs into pSB4A3
  • competency prep on RP437
  • transform RP437 and RP3087 with GFP
  • transform R0100+motB, C0051 (from registry plate) and pSB4A3 into DH5a

Jon

  • use Pat's program to create a map of the IIR+motB part in pSB1A2 and pSB4A3
  • restrict R0051 and motB, and run on gel
  • make LB AND SOB

Chris


Joel

  • Inoculate 6ml of inducible, 2ml constitutive, 2ml DH5-alpha at 11AM.
  • Plate reading on 9hr cultures at 8PM and 24 hr cultures at 10PM.
  • Read Drew Endy's plate reading assay protocals Endy lab page
    • inoculate 2ml of inducible (I14033 and I14032) and 2ml of constitutive.

Ralph

  • Plate read (Pat's Lab) see above
  • Read Drew Endy's plate reading assay protocals Endy lab page

Lane

  • Innoculate colonies from pLacIq+B0030/31/34+C0012 plates
  • ?competency prep on RP437?
  • Transform I14032+E0240 into RP437

Deepti

  • stuff
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