IGEM:Penn State University 2006/calendar/2006-7-5
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Wednesday
Tony
To do today
*e-mail Randy about R0051 and E. coli strain *make sequencing primers (gene-walking and reverse primers) *help Lane with mini-preps, restrictions, gel purification, etc.- assemble LaqIq + motB construct
Accomplished from yesterday
*Sequencing files obtained, examined
Jon
To do today
- restrict (4h) IIR, E0422, E0432, E0434 and run gel
- resubmit primers and IIR+motB and motB for sequencing
- annoy Lane by not inquiring about 96 well plate
Accomplished from yesterday
- miniprep P0451 (from 2005 plate), IIR (from cold room plate), and R0010.motB.B0015 (from successful ligation)
Chris
To do today
*
Accomplished from yesterday
*
Joel
To do today
*inoculate at 11 AM for plate reading at 7PM. Plate reading for 24 hour culture.
Accomplished from yesterday
*
Ralph
To do today
*
Accomplished from yesterday
*
Lane
To do today
*mini-prep LacIq constructs *restrict, ligate, transform (LacIq, B0015+MotB) *innoculate Mary's quorum constructs *do sequencing stuff
Accomplished from yesterday
*Restricted, gel purified, ligated, transformed Mary's GFP+AAV tagged quorum constructs