IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/04/01

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DNA cloning with lyophilized bacteria

Our matrix were 2 mixed culture of lyophilized bacteria cells (from National Institute of Chemistry) which were resuspended in water and sonified. We attempted to clone 2 genes:

  • CtfAB and
  • BdhA

2 concetnrations of each culture were prepared:

  • original concentration
  • 10x dilluted

PCR was conducted accordingly to our protocols.

The PCR was not successful as proven by agarose gel electrophoresis.

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