IGEM:Stanford/2009/Notebook/Marys iGEM Notebook/2009/08/05
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2 The IPP-->B-carotene-->RA Model
The process above can be devided into two procedures:
2.1 Modeling on the production of B-carotene
2.1.1 Yield output of B-caroteneWe found the B-carotene distribution in yeast in [1]. Below is an important form as to the issue.
2.1.2 Production rate of B-caroteneAs this is a really long process....Basically, the velocity is mainly dependent on the most time-consuming reaction in the whole chain. Thus, Leon and I looked for the kcat values of different enzymes in the procedure, as shown in the cart below: Apparently, cyclization of Lycopene is the slowest reaction, as the concentration of enzymes are approximately in the same level. 2.2 Modeling on the B-carotene-->RA processThe production of RA is mainly based on a chain of two catalyzed reactions, as shown in the graph below: Neither Retinal nor RA has any other degrading process in E.coli. Degrading rate of B-carotene is 9.769e-9 s^-1. Other paremeters we use in this model are shown in the form below: 2.3 Simulation and Analysis
References[1] High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2007, p. 4342–4350 Daily WorkGoals
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