IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/04
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Day 2The incubated cells grown well overnight giving a lot of colonies except for 1.E witch gave only one colony We picked single colonies and put them in 10 ml LB with , Kanamicin 50mg/mL Antibiotic for 1.A and 1.E and Ampicilin 100 mg/mL for 1.B 1.C 1.D Then incubated and agitated the tubes at 37ºC for 22 hours We did a Transformation for the RBS and terminator needed for our constructions We followed the same transformation protocol as Day 1 And plated 100μL of the transformants (not 50μL regarding the poor results of the plate 1.E ) in LB + ampicilin 100mg/mL We then incubated the two plates 37ºC for 19 hours
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