IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/11
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Day 7Today we received the package from our team sponsor Thermo scientific[1] We got the Fastdigest® [2]restriction enzymes we ordered We also got a 250 GeneJET™ Plasmid Miniprep Kit [3] The plates put to grow overnight gave poor results no more than 10 colonies in each plate We decided to try to regrow the transformants put to freeze on day 3 [4]and to do the low copy plasmids miniprep procedure on the cultures .
The tubes were put to incubate 16h at 37°C
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