IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/11

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Day 7

Today we received the package from our team sponsor Thermo scientific[1]

We got the Fastdigest® [2]restriction enzymes we ordered

We also got a 250 GeneJET™ Plasmid Miniprep Kit [3]

The plates put to grow overnight gave poor results no more than 10 colonies in each plate

We decided to try to regrow the transformants put to freeze on day 3 [4]and to do the low copy plasmids miniprep procedure on the cultures .


We put them and Two XL1 blue 1.B colonies on two 5ml LB + Ampicilin 100μg/mL except for 1.A in LB + Kanamicin 50μg/mL

The tubes were put to incubate 16h at 37°C




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