IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/12
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Day 8Every tube grown well overnight except for the two 1.E tubes wich are clear after centrifugation we confirmed that nothing grew on the two 1.E tubes We did a miniprep for the other tubes , We used our new GeneJET™ Plasmid Miniprep Kit [1] We checked the samples concentration with the nanodrop [2] We got better results than with the QIAGEN® kit We decided to run an agarose 1% gel electrophoresis to confirm what we got on the samples Expected sizes : 1.A = 4.6 kb 1.B = 2.1 kb 1.C = 2.6 kb 1.D = 2.8 kb 5.A = 2.1 kb 5.B =3.3 kb On this gel we can see that there is a purity problem on 1.C sample and that 1.B samples are out of place , near the 3.5 kb marker tommorow we will run a gel after restriction to identify the problem with 1.B and 1.C samples |