IGEM:The Citadel/Notebook/Notes Brian/06-24-10

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  • (12:30pm) Plated 100μL of cells containing the following parts at the specified concentrations on LB-Miller plates w/ Amp antibiotic:

Original cell solutions diluted w/ LB Lennox broth instead of LB Miller broth (Concentration values listed are relative to the original cell solution concentration) (All plates placed in 37°C incubator at 2:13pm.)

    • BBa_R0062 [50%], [25%]
    • BBa_E0240 [50%], [25%]
    • BBa_R0063 [50%], [25%]
    • BBa_B0015 [50%], [25%]
    • BBa_R0040 [50%], [25%]
    • BBa_J23102 [50%], [25%]
    • BBa_C0160 [50%], [25%]
    • BBa_J45200 [50%], [25%]
    • BBa_F2620 [50%], [25%]
    • BBa_F1610 [100%], [50%], [25%]
    • BBa_I0462 [100%], [50%], [25%]
    • BBa_J45120 [100%], [50%], [25%]
  • Made 22 LB-Miller plates w/ 50μg/mL Kan antibiotic
  • (3:37pm) Inoculated 10 5mL overnight cultures with the following part-laden E.coli:
    • BBa_J23102
    • BBa_R0062
    • BBa_F2620
    • BBa_R0040
    • BBa_R0063
    • BBa_B0015
    • BBa_C0160
    • BBa_J45200 (we are not 100% confident that this colony was introduced to the correct part. Confirm sequence soon.)
    • BBa_E0240
    • BBa_F1610
  • Plated
    • BBa_J23102
    • BBa_R0062
    • BBa_F2620
    • BBa_R0040
    • BBa_R0063
    • BBa_B0015
    • BBa_C0160
    • BBa_J45200
    • BBa_E0240
    • BBa_F1610
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