IGEM:The Citadel/Notebook/Notes Brian/07-21-10

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Biobrick construction map:

1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + pSB3T3 [E,P] = ~J02F10 (T)

  • J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly.

2.0 I746350 (A) + B0015 (C, ex A) + pSB3T3 = ~I50B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

2.1 ~I50B15 (T) + I746360 (A) + pSB3C3 = K274370 (C)
2.2 ~I50B15 (T) + I746361 (A) + pSB3C3 = K274371 (C)
2.3 ~I50B15 (T) + I746364 (A) + pSB3C3 = K274374 (C)
2.4 ~I50B15 (T) + I746365 (A) + pSB3C3 = K274375 (C)
3.0 I746351 (A) + B0015 (C, ex A) + pSB3T3 = ~I51B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

3.1 ~I51B15 (T) + I746360 (A) + pSB3C3 = K274380 (C)
3.2 ~I51B15 (T) + I746361 (A) + pSB3C3 = K274381 (C)
3.3 ~I51B15 (T) + I746362 (A) + pSB3C3 = K274382 (C)
3.4 ~I51B15 (T) + I746364 (A) + pSB3C3 = K274384 (C)
4.0 I746352 (A) + B0015 (C, ex A) + pSB3T3 = ~I52B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

4.1 ~I52B15 (T) + I746361 (A) + pSB3C3 = K274391 (C)
4.2 ~I52B15 (T) + I746362 (A) + pSB3C3 = K274392 (C)
4.3 ~I52B15 (T) + I746364 (A) + pSB3C3 = K274394 (C)
4.4 ~I52B15 (T) + I746365 (A) + pSB3C3 = K274395 (C)
5.0 F2620 (A) + K274382 (C) + pSB3T3 = ~F20K82 (T)
5.1 ~F20K82 (T) + E0240 (A) + pSB3C3 = ~F20K82E40 (C)
5.2 ~J02F10 (T) + ~F20K82E40 (C) + pSB3A3 = ~J02F10F20K82E40 (A) [QS & ST tester]
6.0 B0034 (A) + C0160 (C, ex A) + pSB3T3 = ~B34C60 (T)
7.0 ~B34C60 (T) + B0015 (C, ex A) + pSB3A3 = K218026 (A)

Performed restriction digest on:

1. J23102 - E,P
2. F1610 - X,P
3. B0015 - E,P
4. I746350 - E,S
5. I746360 - X,P
6. I746362 - X,P
7. I746361 - X,P
8. I746364 - X,P
9. I746365 - X,P
10. F2620 - E,S
11. E0240 - X,P
12. B0034 - E,S
13. C0160 - E,P


Nanodrop Specs:

1.1 = 37ul; 131.53 ng/ul
1.2 = 41ul; 255.00 ng/ul
2.1 = 43ul; 135.54 ng/ul
2.2 = 42ul; 152.44 ng/ul
R0063 = 47.60 ng/ul
B0015 = 139.80 ng/ul
J45200 = 273.29 ng/ul
F2620 = 110.27 ng/ul
I0462 = 92.14 ng/ul
J45120 = 208.75 ng/ul
R0062 = 100.64 ng/ul
J23102 = 67.17 ng/ul
E0240 = 76.85 ng/ul
C0160 = 98.77 ng/ul
R0040 = 66.56 ng/ul
F1610 = 126.03 ng/ul
I20260 = 22.56 ng/ul
I746394 = 137.42 ng/ul
P1010 = 24.35 ng/ul
I746395 = 183.81 ng/ul
I746370 = 154.2 ng/ul
I746380 = 205.2 ng/ul
I746392 = 168.2 ng/ul
I746374 = 239.8 ng/ul
I746391 = 166.1 ng/ul
I746381 = 174.2 ng/ul
I746382 = 175.4 ng/ul
I746384 = 190.1 ng/ul
I746375 = 228.3 ng/ul
I746371 = 68.3 ng/ul
P1010(2) = 19.7 ng/ul
B0034 = 83.9 ng/ul
I746350 = 72.4 ng/ul
I746351 = 102.2 ng/ul
I746352 = 88.8 ng/ul
I746360 = 82.3 ng/ul
I746361 = 109.4 ng/ul
I746362 = 88.5 ng/ul
I746364 = 79.9 ng/ul
I746365 = 85.7 ng/ul

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