IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/31
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Tuesday 31st August:
1) pACYC Plasmid - 35μL Nco1 - 1μL Buffer 3 - 4μL BSA (x100) - 0.4μL 2) pSB1C3 Plasmid- 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL 3) pga Promoter Plasmid - 10μL EcoR1 - 2μL EcoR1 buffer - 2μL Spe1 - 2μL BSA (x20) - 1μL H2O - 3μL 4) csrB Plasmid - 10μL EcoR1 - 2μL EcoR1 buffer - 2μL Pst1 - 2μL BSA (x20) - 1μL H2O - 3μL 5) Part BBa_E0040 (GFP) Plasmid - 10μL EcoR1 - 2μL Spe1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL
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