IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/02

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

Thursday 2nd September

  • The overnight digestions were checked using gel electrophoresis [[1]].
  • The gel was set up as follows starting from the bottom:

1) GFP plasmid from the lab. 2) Part BBa_E0040. 3) Ladder.

Image:UVP00449.JPG


  • Both bands in lane 2 were removed using the gel extraction kit.[[2]]


* Ligation of pgaA promoter, Part BBa_E0040 and pSB1C3:

 pgaA promoter - 2μL
 Part BBa_E0040 - 2μL
 pSB1C3 - 2μL
 T4 DNA ligase - 1μL
 T4 buffer (x10) - 2μL
 H2O - 11μL
  • Ligation failed as shown by the bellow gel:

Image:UVP00452.JPG‎

  • The ligation is in lane one. The three bands show that ligations was not successful.




Personal tools