IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/09

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Thursday 9th September

  • The cut promoters were ligated with part BBa_E0040 which has been cut with EcoR1 and Xba1, using the following method for each promoter:


Substance (μL) Tube 1 Tube 2 Tube 3 Tube 4
BBa_E0040 1 2 5 2
Promoter 1 5 2 2
Ligase 1 1 1 0
Buffer 1 1 1 1
H2O 6 1 1 5


  • The different methods were used to see which volumes produced the best results.



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