IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/12
From OpenWetWare
Jump to navigationJump to search
iGEM Project name 1 | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
dspB TrackMarianne and VickiPCR genomic preps (PCR I)Start from the beginning again with the A. pleurpneumoniae strains. PCR out dspB from the strains, dilute product, PCR dspB again, extract dspB from gel (purification). Strains:
Protocol: Common protocols for PCR
2 Master Mixes:
PCR Cycles:
Start: 1316 Gel VerificationProtocol: gel verification protocol in iGEM training manual Gel orientation:
*Note: the second layer are Jason's samples - he is testing whether his PCR from last day worked or not Machine conditions: 110V, 45 min, 0.5X TBE Buffer |