IGEM:UC Berkeley/2006/Digestion

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Digestion of a Mini-prep DNA (35 ul scale)

  1. 13 uL water
  2. 3.5 uL buff(3)
  3. 3.5 uL BSA
  4. 1 uL Enzyme 1
  5. 1 uL Enzyme 2
  6. 15 uL DNA

  35 

INCUBATE AT 37 degrees for 2 hours!


Digestion of a Mini-prep DNA (20 uL scale)

  1. 10 uL DNA
  2. 7 uL water
  3. 2 uL 10x buffer
  4. 0.5 uL Enzyme 1
  5. 0.5 uL Enzyme 2

  20 

Scaled up digestion of mini-prep

  1. 14uL H2O
  2. 4uL NEB Buffer 2
  3. 20uL DNA
  4. 1uL restriction enzyme
  5. 1uL restriction enzyme

   40 


Digestion of PCR Product

  1. Elute 108uL of water
  2. 12 uL of 10X NEB Buffer 2
  3. 1 uL Enzyme 1
  4. 1 uL Enzyme 2

  120 uL
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