IGEM:UC Berkeley/2006/KCMSmallScale

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  1. Grow cells in ~4 mL LB until cloudy
  2. Put on ice
  3. Transfer 1mL into an eppendorf tube on ice, let cool
  4. Centrifuge full speed for 30 sec, toss out supernatant
  5. Resuspend in 90uL of TSS solution
  6. Add 10uL KCM
  7. Add 1uL plasmid DNA
  8. Let sit on ice for 10min, heat shock 90 sec at 42, incubate and/or plate
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