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First Video-conference with Edinburgh
Teams Experimental advances:
Both teams have been working on the same Firefly Luciferase mutation to get the red-shift emission output. However, the distance between the promoter and the RBS is different in each team construction.
Before, both teams noticed the similarity of our projects. The Edinburgh’s advisor kindly sent us LuxAB and LuxCDE PCR products and a plasmid containing the YFP protein:Lumazine. Both teams have failed with LuxCDE.
Edinburgh team is working with LovTAP, the induction with IPTG doesn´t seem to work well. Our team sent to Mr. Gene a new-LovTAP design considering some improvements reported by the Lausanne team and others based on Devin’s advices, the creator of LovTAP. Besides, our team is using a native E.coli blue inducible promoter but the induction seems to be very weak.
Both teams have failed to get the cph8 chimerical photoreceptor and the producing chromophore PCB genes of the Parts distribution.