IGEM:UNAM-Genomics Mexico/2009/Notebook/Collaborations/2010/08/30

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Shipment, more details

Hi Edinburgh Team!

We have been working on the shipment issues and updating our logbooks, in order that you get totally familiarized with our parts. The shipment was ready on Friday but we were notified that the airplane is going to fly on Monday. We are sending the shipment using DHL. The tracking number of the shipment is 2 72 234 6745.

We are sending the following parts:

•Plasmid standard vector from Registry of Standard Biological Parts with an insert consisting of a blue light inducible promoter.*

•PCR amplified product containing green light photosensor CcaS and CcaR response regulator.

•PCR amplified product containing blue light photosensor LovTAP.**

•PCR amplified product containing a YFP protein, LuxY.

•PCR amplified product containing light emitting luciferase genes LuxAB from Vibrio Fischeri.

•Plasmid J61002 (Registry of Standard Biological Parts) harboring the blue light photosensor LovTAP ligated to promoter J23117.**

•Plasmid J61002 (Registry of Standard Biological Parts) harboring the blue light photosensor LovTAP ligated to promoter J23105.**

•Plasmid J61002 (Registry of Standard Biological Parts) harboring the blue light photosensor LovTAP ligated to promoter J23114.**

•Plasmid J61002 (Registry of Standard Biological Parts) harboring the blue light photosensor LovTAP ligated to promoter J23102.**

-*The ligation of the blue light inducible promoter with GFP protein has not been confirmed yet. It seems to be incorrect.

-**LovTAP ligation with plasmid pSB1C3 was incorrectly done; we cultured the cells in the wrong selective medium. But we are sending a PCR product from Mr. Gene DNA sequence and four ligations with two weak, one medium and one strong promoter.

Mariana´s luciferase didn’t glow. She tested the wt and mutated S284T luciferases with the RBS changed, and none emitted bioluminescence. She is now transforming the wt Luciferase with the RBS unchanged, in order to know if the RBS change is the cause of the results.

The gels that we made from the PCR amplified products that we are sending are in the next link:

http://openwetware.org/wiki/IGEM:UNAM/2009/Notebook/Modeling_logbook_Claudia/2010/08/25

The details of LovTAP ligations are in the following link:

http://openwetware.org/wiki/IGEM:UNAM/2009/Notebook/Modeling_logbook_Claudia/2010/08/26

We are still working in those entries; we will update the details of the DNA sequences, hopefully today they will be ready.

Considering the results and advances in our two light emitter systems (firefly and vibrio fischeri), we are worried about finishing them on time; thus we were wondering if you are in touch with Cambridge team, that seems to already have results with bioluminescence reactions. one possibility that we have thought about is to ask them for their glowing bacteria. We were curious to know whether you are trusting on getting your own emmision system or you're also considering this possibility as an emergency alternative. If so, we could start thinking about expanding the collaboration to the Cambridge team and, for instance, characterize their luciferase activity among other cool possibilities. What do you think?

Well, we hope that our parts can be useful for your project and feel free to contact us for any doubts.

Best wishes,

UNAM-Genomics-Mexico team.




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