IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/02

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Test of purification plasmid kit by Roche

  • Pato and I follow the steps described by the protocol's kit, in order to isolate the plasmid pBBRMSC5 which was in E. coli
  • We did an electroforesis with a 1% agarose gel, 80 V X 100 min, 3 microL dye and 5 microL DNA
  The gel looked like there were genome DNA 
  • With the same isoleted plasmid, we did another electroforesis in order to check if the features of it were the cause of the bad result. 125 V X 30 min, 3 microL dye and 10 microL DNA
 The gel show the two bands corresponding to the chromosome, which were charged in two lanes:



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