IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/21

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Doing competent cells

  • Daniel put 5 ml of liquid medium with a BL21's colony to grow since the morning.
  • About 12:30 were picked up 100 micro-litters and passed into 1 litter.
  • The cell grew up until reach OD = 0.1, Miguel said to us that because E. coli replicate

every 30, we just needed to leave them growing 1 hour to reach the neccessary OD = 0.4

  • Once passed 1 hour, we centrifugated at maximum speed for 10 minutes and we descarted the supernatant.
  • We added 1 ml of cool CaCl and vortex it, then we left them in ice all nigth.

Tomorrow we'll finish the procedure.



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