IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/11

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

bjectives

To see if our PCR was correctly inserted into pBBRMCS-5, doing a restriction with the same enzymes used to generate sticky end between the plasmid and the PCR, these enzymes are apaI and sacI.

Transformation of pBBRMCS-5

Because apaI doesn´t work with dcm methylase which is present in sp. DH5alfa, I transformated the plasmid into sp. BL21 whic doesn´t have dcm. Tomoorrow I will do the isolation from the colonies resulting from today transformation and the double digestion by apaI and sacI.

I did 3 transformation reactions:

1. DNA taken from the second lane.

2. DNA taken from the fifth lane.

3. No DNA.

This gel comes from here

The transformation was did according to this protocol



Personal tools