IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/30
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1. Continuing the plasmid's stability experiment. Dilutions and plaqued from the 74 hours culture and took of 100 colonies from 60 hours dilutions.
1. Vortex the 1 ml culture. 2. Take 100μl of the previous culture and put them in 900 μL of liquid medium of antibiotic. This is the first dilution. 3. Vortex 30 times the dilution. 4. Repeat the steps 2 and 3 until reach the desired dilution.
I did it until reached the 5th dilution, because the dilutions of previous hours were too many to properly obtain 100 colonies. Then I plaqued 100 μL of the dilutions 3,4, and 5.