IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/03
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1. I put to grow in 1 mL of liquid PY with CaCL2 a big pellet of: E. coli S17 carrying the pBBR1MCS-5(donor), the cultive was left 4 hours. It's also neccesary to have R. elti CE3 grown in this moment because the R. etli duplication is more slowly, Miguel gave me one cultive of grown R. etli.
2. In a steril test tube I put 100μL of the grown E. coli S17, 200μL R. etli CE3.
3. I took all the 300 μL from the last test tube and I put it in the center of a petri dish with solid PY(CaCl2), it was left over nigth at 30°C