IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/09/02

iGEM Project name 1

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Abstract Repeating the standardization Main Standardization Digestion results In the following electrophoresis gel (120V X 45min) Lanes: 1. Ladder 500 bp. 2. PCR Digestion. 3. pBBR1MCS-5 digestion. 4. pBBR1MCS-5 plasmid The remaining lanes are not of interest for this analysis The PCR is not visible in this gel but a I think its not enough concetration to see it, however a problem could have arise during the PCR cleaning Ligation Regardless the problem of the PCR in the gel I did 3 ligations with these amounts: Compound Rx 1 Rx 2 Rx 3 (control) H20 3 3 6 Buffer T4 10x 1 1 1 Vector 1 2 2 PCR product 4 3 0 T4 DNA Ligase 1 1 1 I left the rections 5 hours Transformation I did the transformation of the previous ligations according to this protocol. At the end I had 4 petri dishes, three ligations and one medium control