IGEM:UNAM/2009/Notebook/IGEM project/2011/06/29

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Transformation of competent cells "Escherichia coli"

  • Today I transform plasmids with iGEM parts in Escherichia coli.

Method to transform

  • 1. Unfreeze the competent cells.
  • 2. Put 1 μl of DNA in the eppendorf tube of competen cells and wait 20 minutes.
  • 3. Put the competent cells with DNA in the Termo to 42 °C for one minute.
  • 4. Leave the competent cells five 5 minutes in ice and put 1 ml of liquid LB.
  • 5. Leave the tubes for one hour to 37 °C.
  • 6. Centrifuge the tubes for 30 seconds to 13 mil rpm.
  • 6. Trow supernant and shake the cells in vortex.
  • 7. Take the drop of the tubes and put in the petri dish.
  • 8. Scatter the cells with the bail.

Petri dishes with iGEM parts:

Petri dish control 1- LB medium with Ampicillin 1μl/ml.
Petri dish J23103- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23103.
Petri dish J23104- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23104.
Petri dish J23105- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23105.
Petri dish J23106- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23106.
Petri dish J23107- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23107.
Petri dish J23108- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23108.
Petri dish J23109- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23109.
Petri dish J23110- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23110.
Petri dish J23111- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23111.
Petri dish J23112- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23112.
Petri dish J23113- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23113.
Petri dish J23114- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23114.
Petri dish J23115- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23115.
Petri dish J23116- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23116.
Petri dish J23117- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23117.
Petri dish J23118- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23118.
Petri dish J23119- LB medium with Ampicillin 1μl/ml + E. coli with the iGEM part J23119.

Petri dish control 2- LB medium with Kanamycin 1μl/ml.
Petri dish I20260- LB medium with Kanamycin 1μl/ml + E. coli with the iGEM part I20260.


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