IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/05/11

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Objective: Research the activity constant of the luminescence reaction (k_kat) by Luciferase from fire fly

In the constructed light-switch from yeast:

K_cat =4.99 E+02 nM/h  [1]


In the heterologous expression of luciferase in mouse kidney cells:

K_cat= 0.04  moles of oxyluciferin / ( moles of luciferase *seg)  [2]


References

1. Sorokina, O., Kapus, A., Terecskei, K., Dixon, L. E., Kozma-Bognar, L., Nagy, F., et al. (2009). A switchable light-input, light-output system modelled and constructed in yeast. Journal of biological engineering, 3, 15. doi: 10.1186/1754-1611-3-15


2. Ignowski, J. M., & Schaffer, D. V. (2004). Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells. Biotechnology and bioengineering, 86(7), 827-34. doi: 10.1002/bit.20059.




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