IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/04
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Modeling & WetLab Meetings
Stoichiometry of Luciferase activity.
a[Luc] + b[ Luc-ina] + c + d[ATP] -> z[Luc] + y [oxyLuc-ina] + w[C02] + u[AMP] + v[PPi]
We use that formula to know which is the limitant reactant and the optimum concentration.
To achieve a controlled model we use single copy plasmid or low copy number.
LuM= Kc_cat * LUC * (Scyt/(Km+Scyt)) *RLV
K_cat= [Constant of Activity]
Scyt = [Cytosolic Luciferase]
Km= Michaelis-Menten constant.
RLU= Relative Light Unit
LuM= Number of moles, which react in measure, this is by colony.
With this formula we can achieve the values for light emission and efficiency of our system. We need:
To get unidirectionality we purpose a system with optical fiber and aisolated systems.
Each beaker is coated by aluminium to focus photons to exit. This design is simple, we will change datails in sizes of elements and positions.