IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/11

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

May 11th, 2010

Modeling

Luciferase

  • We are working for Luciferase model.
  • The units will be measured from SI, not RLU; this is to standardize inputs and outputs.
  • The emission need be measured in vitro vs in vivo because there are differences for cytosolic Luciferin and free luciferin.
  • In literature data shows enzyme saturation in mice for inoculations with luciferin:

1.55mM-> Cytosolic luciferin

134 μM-> Free luciferin.

-For this we need to measure the relation between substrate-enzyme and luciferin in-out cytosolic.

A [Luc-ina]cyt = [Luc-ina]RECY + K[Luc-ina]NON-CYT + K’[Luc-ina]cyt – R[Luc-ina]cyt – D[Luc-ina]cyt

K = Constant of permeability of influx.

K = Constant of permeability of efflux.

R= Constant of activity.

D= Constant of degradation.

In literature the constant of activity for Luciferase = 4.83 e-6 mol/seg.

  • For these constants we need:

-Degradation rate

-Reaction rate (Luciferase activity).

-Rates of permeability.

This equation will serve to maximize the activity of luciferase and light creation.

Regenerating luciferin enzyme

Image:Model.11.May.2010.RecycleoxyLuciferin.jpg

Other stuff

  • Search for kits of Luciferin inoculation.



Personal tools