IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/11
|iGEM Project name 1|| Main project page|
Previous entry Next entry
May 11th, 2010
1.55mM-> Cytosolic luciferin
134 μM-> Free luciferin.
-For this we need to measure the relation between substrate-enzyme and luciferin in-out cytosolic.
A [Luc-ina]cyt = [Luc-ina]RECY + K[Luc-ina]NON-CYT + K’[Luc-ina]cyt – R[Luc-ina]cyt – D[Luc-ina]cyt
K = Constant of permeability of influx.
K = Constant of permeability of efflux.
R= Constant of activity.
D= Constant of degradation.
In literature the constant of activity for Luciferase = 4.83 e-6 mol/seg.
-Reaction rate (Luciferase activity).
-Rates of permeability.
This equation will serve to maximize the activity of luciferase and light creation.
Regenerating luciferin enzyme