IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/11
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May 11th, 2010ModelingLuciferase
1.55mM-> Cytosolic luciferin 134 μM-> Free luciferin. -For this we need to measure the relation between substrate-enzyme and luciferin in-out cytosolic. A [Luc-ina]cyt = [Luc-ina]RECY + K[Luc-ina]NON-CYT + K’[Luc-ina]cyt – R[Luc-ina]cyt – D[Luc-ina]cyt K = Constant of permeability of influx. K = Constant of permeability of efflux. R= Constant of activity. D= Constant of degradation. In literature the constant of activity for Luciferase = 4.83 e-6 mol/seg.
-Degradation rate -Reaction rate (Luciferase activity). -Rates of permeability. This equation will serve to maximize the activity of luciferase and light creation. Regenerating luciferin enzyme Other stuff
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