IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/25
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25th May 2010RepresilatorA brief review to compare
A loop negative system.
Degradation of the mRNA, protein and the rate of transcription. Perspectives for our system
Limitants -> lifespan of E. coli?, luciferin?. "If is all on, we expect, that stuck there, but if one system turn off, maybe there is a stochastic component". LOVTAP SystemLifespan TetR
[math]\displaystyle{ δTetR= β - αTetRο }[/math] We require a formula: [math]\displaystyle{ F([TetR]) = Pops }[/math] But what happen with the production of endougenos TetR?, exists?.
Green emissionInput Pops -> Photons. τ → ∞ -> [math]\displaystyle{ [14-CHO]=k }[/math]
Again we need to propose a formula: [math]\displaystyle{ [LuxAB]=Pops=N*f(Promoter force) }[/math] Pathway[math]\displaystyle{ LuxAB-FMNH }[/math]2[math]\displaystyle{ -O }[/math]2[math]\displaystyle{ -R-CHO -\gt LuxAB + FMN + R-COOH + H }[/math]2[math]\displaystyle{ O + Light }[/math] And the enzymes work in this form (Daniela has the references in German :S): [math]\displaystyle{ Acid M. - }[/math]LuxD[math]\displaystyle{ \gt C }[/math]13[math]\displaystyle{ H }[/math]27[math]\displaystyle{ -COOH - }[/math]LuxE[math]\displaystyle{ \gt C }[/math]13[math]\displaystyle{ H }[/math]27[math]\displaystyle{ Co-Amp - }[/math]LuxC[math]\displaystyle{ \gt 14-CHO }[/math] Here we see:
Modelling with a Michael-Menten paradigmPerspectivesIt is relevant?:
Can we assume Micahel-Menten equation? To find list
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