IGEM:UNAM LCG/2009/Notebook/Hydrobium etli/2011/09/29
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pBBR1MCS-5 Double digestion
I made a double digestion with PstI and EcoRI of the plasmid pBBR1MCS-5 that Paulina extracted.
I need this plasmid digested to ligate all the anderson collection promoters.
I do not exctly know the plasmid pBBR1MCS-5 concentration or even if the extraction was good, but Paulina says it was, I have to ask her for the gel image.
I made 4 reactions, 3 were from 3 different pBBR1MCS-5 extractions or samples and one was as a control from a part that Fabricio had already made and went well, this part has a RFP.
Reactive amounts and details