IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12
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1. Restriction digest of pTrc99A-AID with HindIII and NcoI.
2. DNA electrophoresis and isolation of DNA encoding AID from proper band.
3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).
4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.
5. DNA ligation (pMPM-T5+AID vector)