IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19

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University of Warsaw iGEM Team 2008 Main project page
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1. PCR - AID for translation fusion

Primers:

AIDlNrH
AIDpLinB

Template DNA: pTrc99A-AID

Annealing temperature: 55 °C

Annealing time: 60 sec

20 cycles


2. PCR - T7 RNA-polimerase for translation fusion

Primers:

T7lLinkB
T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles


3. PCR - T7 RNA-polimerase for transcription fusion

Primers:

T7lRBSHi
T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles


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