IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/20

From OpenWetWare

Jump to: navigation, search
University of Warsaw iGEM Team 2008 Main project page
Previous entry      Next entry

1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands.

2. PCR - translation fusion: AID + T7 RNA-polimerase

Primers: AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion

Annealing temperature: 55 °C

Annealing time: 4 minutes

20 cycles

3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)


Personal tools