IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/20
From OpenWetWare
Jump to navigationJump to search
 University of Warsaw iGEM Team 2008
|
 Main project page Previous entry Next entry
|
|
1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSal Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase) | |
