IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/20

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University of Warsaw iGEM Team 2008 Main project page
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1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands.

2. PCR - translation fusion: AID + T7 RNA-polimerase

Primers: AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion

Annealing temperature: 55 °C

Annealing time: 4 minutes

20 cycles

3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)

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