IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/29

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1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB

2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose

3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB

4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG

5. Wait for 5 hours

6. OD measurment

7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + riphampicine)


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