IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/03

From OpenWetWare

Jump to: navigation, search
University of Warsaw iGEM Team 2008 Main project page
Previous entry      Next entry


1. Gel electrophoresis (PCR products) and isolation from proper bands

2. Digest with HindIII and SalI

3. Digest of pMPM-T5 + AID with HindIII and SalI

4. Clean-up products of p.2 and p.3 reactions

5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion)

6. Transformation of E.coli TOP10 with ligation product

7. Transformants onto LB+tetracycline

8. Digest of pMPM-T5 + transcription fusion with EcoRHI and HindIII

9. Use of T4 polymerase for blunting

10. Gel electrophoresis

11. Isolation from proper band

12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polimerase)

13. Transformation of E.coli TOP10 with ligation product

14. Transformants onto LB + tetracycline




Personal tools